TSA Multi-colour Immunofluorescence System Kit (4-Color)

TSA Multi-colour Immunofluorescence System Kit (4-Color)

Author: ApexBio

shortsummary:

TSA 4-colour Immunofluorescence kit，used for signal amplification of in situ hybridization (ISH), immunohistochemistry (IHC), immunocytochemistry (ICC) and other related experiments.

Concanavalin

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In stock

halt protease inhibitor cocktail

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Blue ice

description:

Tyramide Signal Amplification (TSA) technology can be used to detect low-abundance targets in tissue and cell immunofluorescence (IF), immunochemistry, in situ hybridization (ISH), etc., increasing signal sensitivity by about 100-fold. The HRP-labeled secondary antibody can activate the fluorescently labeled tyramide molecules in the presence of H2O2, and convert the labeled tyramide into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues in the vicinity of the reaction site. The labeling process is rapid (less than 10 minutes), and the deposition marks can be viewed directly under a standard or confocal microscope. TSA fluorescence kits can also be used in combination with traditional immunofluorescence methods for multicolor imaging, or two or more tyramide reactions can be performed sequentially to label different targets on one sample (for example, in this kit, three different fluorescent tyramide molecules were employed). The use of TSA reagents can significantly improve signal sensitivity while maintaining the specificity and resolution ratio compared to normal experiments, in addition, TSA reagents can significantly reduce the consumption of primary antibodies or probes.

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APExBIO Technology LLC is a premier provider of Small Molecule Inhibitors/Activators, Compound Libraries, Peptides, Assay Kits, Fluorescent Labels, Enzymes, Modified Nucleotides, mRNA synthesis and various tools for Molecular Biology. We carry a broad product line in over 18937 different research areas such as cancer, immunology, neurosciences, apoptosis and epigenetics etc. Based in USA (Houston, Texas), we have been serving the needs of customers across the world.